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Cellular Ultrastructure and Protein Localization

The localization of many disease biomarkers within cells, particularly those in endomembranes such as the Golgi apparatus, are not well understood.  pan-ExM allows biologists to determine the 3-D molecular organization of proteins at sub-organelle scale (~15nm) and locate them precisely in the larger context of cellular ultrastructure.

pan-ExM antibody labeling of the Golgi complex

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pan-ExM dual protein and lipid pan-staining of cells

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pan-ExM ultrastructural context with light microscopy 

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panluminate has experience processing cells grown on coverslips or suspended in solution, from common research cell lines (e.g., HeLa, COS-7, U2OS), to embryonic stem cells, hematopoietic cells, whole embryos (e.g., zebrafish) and even whole model organisms (e.g., Xenopus Laevis developmental stage 50).

Translational Research

Biomarkers in complex tissue (e.g., the tumor microenvironment, fibrotic lung tissue, kidney tissue in patients with glomerulonephritis etc.) all manifest at the subcellular level. To understand the minute molecular and ultrastructural phenotypic signatures at the early onset of humanity's most pressing diseases, we must be able to visualize the 3D organization of complex tissue at the nanoscale, as well as correlate the distributions of specific cells (e.g., immune cells), biomarkers, and drugs/ biologics (e.g., Antibody Drug Conjugates) to their 3D ultrastructural context.

pan-ExM dual protein and lipid staining of brain tissue

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pan-ExM applied to mouse kidney

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panluminate has experience processing brain, lung, and kidney tissue in model organisms of neurodegeneration (e.g. Alzheimer's disease), fibrosis, and acute-kidney injury respectively. We also developed a new way to preserve, process, and localize biologics in patient-derived xenograft (PDX) tissue. With a new optimized pipeline, we help companies in the drug preclinical development stage visualize their biologics and biomarkers of interest in the 3D ultrastructural context of tissue.

Connectomics Sample Prep

In brain tissue processed with panluminate technology, all neurites (dendrites, axons), cellular organelles, synaptic densities, and membrane boundaries are revealed with a proprietary contrast that resembles heavy metal stains used in electron microscopy. Proof-of-concept image segmentation confirms that whole neurons and their synaptic connections can be traced. Combined with immunofluorescence, we were able, for the first time, to molecularly annotate neural circuits acquired on a light microscope. 

pan-ExM 3-D imaging of cellular markers in brain tissue

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panluminate provides sample prep services for labs and companies that either conduct large-scale connectomics projects or develop neuronal tracing algorithms for connectomics analysis. 

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