A radical 10,000x volumetric sample expansion
We developed pan-ExSt, a patented two-step polymerization process which expands samples a record
10,000x volumetrically or 25x linearly while preserving total protein content and relative nanoscopic distances between proteins.
This expansion allows for variable levels of resolution ranging from the tissue scale (~250 nm at 1x) to the nanoscale (~10 nm at 25x) and provides clearing and separation between proteins, enabling detailed probing of each molecule.
pan-staining reveals ultrastructural context
Next, we apply pan-staining, a proprietary contrast that details all cellular organelles and compartments on a fluorescence microscope all at once, for the first time in light microscopy.
pan-staining is an optical analog to the heavy metal stains used in Electron Microscopy (EM). Prior to pan-staining, investigators had to rely on cumbersome EM for ultrastructural analysis and the diagnostics of multiple diseases.
pan-ExSt localizes biomarkers in context
pan-ExSt integrates protein labeling with ultrastructural analysis, which is beyond the reach of any current imaging modality
This tool allows for the precise localization protein biomarkers in ultrastructural context, uncovering the cellular mechanics that drive disease mechanisms and drug responses at the fundamental protein cluster level (~10 nm).
pan-ChroMax enables multimodal DNA, RNA and protein labeling
Going beyond proteins, pan-ChroMax combines DNA labeling, RNA FISH, and antibody labeling to illuminate the nanoscale organization of chromatin and transcription.
By resolving transcription condensates, pan-ChroMax
has helped scientists achieve breakthroughs in understanding gene activation and regulation.
pan-RefleXion reveals cells with the naked eye
pan-RefleXion takes the concepts of expansion and pan-staining to their ultimate conclusion, revealing cells with the unaided human eye.
Though ahead of its time in medical applications, we are at the forefront of this imaging frontier, envisioning a future where rapid diagnostics can be conducted without the need for optical instruments.
Visible HeLa cells expanded and pan-stained with metallic silver
Nature Communications, 07/20